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| 产品名称： | VIII-6G10 |
|---|---|
| 商品货号： | TS214329 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque |
| Cellular Products： | immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in BALB/c mice |
| Comments： | Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina. Spleen cells were fused with NS-1 myeloma cells. The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells. |
| Complete Growth Medium： | RPMI 1640 medium with 25 mM HEPES, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | Fred Hutchinson Cancer Res. Cntr. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Masinovsky B, et al. IL-4 and TNF induce mAb 6G10-recognized expression on bone marrow stromal cells. US Patent 5,206,345 dated Apr 27 1993 Masinovsky B, et al. IL-4 acts synergistically with IL-1 beta to promote lymphocyte adhesion to microvascular endothelium by induction of vascular cell adhesion molecule-1. J. Immunol. 145: 2886-2895, 1990. PubMed: 1698865 |