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| 产品名称： | pACHRGcDNA ACh receptor gamma cDNA |
|---|---|
| 商品货号： | TS214334 |
| Designations： | pACHRGcDNA ACh receptor gamma cDNA |
| GenBank Number： | X03818 |
| Species： | Mus musculus, mouse |
| Depositors： | JP Merlie |
| Vector： | Construct size (kb): 4.71999979019165 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.705000042915344 Tissue: muscle BC3H-1 cell line Gene product: acetylcholine receptor gamma Acrg |
| Insert Size (kb)： | 1.705 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (nt positions according to the published sequence): HindIII--177; EcoRV--344; PvuII--554, 1070; PstI--680, 1085; AvaI--1168; BglI--1332. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; HindIII--3.2, 1.5; AvaI--4.1, 0.6; PstI--3.5, 1.1; BglI--2.9, 1.8. Detects a single EcoRI fragment of 10.8 kb and a single BamHI fragment of 16.5 kb in Southern blots of genomic DNA from BC3H-1 cells. Contains the complete coding sequence (nt 12-1571). Detects a mRNA of 2.0 kb. Oriented such that transcription from the SP6 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377 Yu L, et al. Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression. Nucleic Acids Res. 14: 3539-3555, 1986. PubMed: 3010242 John P Merlie, personal communication |