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| 产品名称： | IgG-2F1 |
|---|---|
| 商品货号： | TS214346 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized against GST-Rab escort protein-1 (REP-1). The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes. It is useful for detecting the level of REP-1.
This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human REP-1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human REP-1 |
| Comments： | Animals were immunized against GST-Rab escort protein-1 (REP-1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes. It is useful for detecting the level of REP-1.
This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Name of Depositor： | JL Goldstein, YK Ho |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170 Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339 Seabra MC, et al. Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell 70: 1049-1057, 1992. PubMed: 1525821 |