Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.

The antibody cross-reacts with CD11b from rat and canine cells.

Spleen cells were fused with NS-1 myeloma cells.

immunoglobulin; monoclonal antibody; against human CD11b

immunoglobulin; monoclonal antibody; against human CD11b

Animals were immunized with human acute monocytic leukemia cells (FAM M6).

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.

The antibody cross-reacts with CD11b from rat and canine cells.

Culture Medium: Modified Dulbeccos medium (see below), 90%; Gamma
globulin-free horse serum, 10%.
DME medium with 4.5 g/L glucose 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Hypoxanthine (10 mM) - Thymidine (1.6 mM) 1 mL
NCTC 135 10 mL
Gamma Globulin-Free Horse Serum (inactivated) 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.