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| 产品名称： | pY3 |
|---|---|
| 商品货号： | TS214417 |
| Designations： | pY3 |
| Depositors： | K Blochlinger |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.8699998855590820 Vector: pY3 (plasmid) Promoters: Promoter Mo-SV LTR Construction: pLG88, pMLTR-1 Marker(s):tetR,hygR Construct size (kb): 5.869999885559082 Features: marker(s): hygR, tetR promoter: Mo-SV LTR replicon: pMB1 enhancer: Mo-MuLV LTR |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 0.9; KpnI--6.0; PstI--4.8, 1.2. Plasmid vector allowing for direct selection for hygromycin B resistance after transfection of eukaryotic cell lines. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Blochlinger K, Diggelmann H. Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol. Cell. Biol. 4: 2929-2931, 1984. PubMed: 6098829 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |