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| 产品名称： | HS-Sultan |
|---|---|
| 商品货号： | TS214422 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 2 Cells contain EBV DNA viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 7 |
| Gender： | male |
| Ethnicity： | Black, African |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hyperdiploid human cell line. The modal chromosome number was 48, occurring in 80% of cells, and the rate of polyploid cells was 3.4%. Three marker chromosomes are common to all cells. M1 and M2 der(14)t(8;14) (q24;q32) were probably derived from the balanced translocation between del(8) (p21.1;p21.3) and N14. The third marker, M3, is ?der(8)t(8;?) (p21.1;?). Most cells had three copies of N7. Both normal X and Y were clearly identifiable. |
| Derivation： | Although deposited with the ATCC as a plasmacytoma cell line, DNA fingerprinting has shown this line to be a derivative of Jiyoye (ATCC CCL-87), a Burkitts lymphoma cell line. |
| Clinical Data： | Black, African male 7 |
| Antigen Expression： | CD11a +; CD19 +; CD20 +; CD28 -; CD38 +; CD49e + |
| Genes Expressed： | immunoglobulin |
| Cellular Products： | immunoglobulin |
| Comments： | The cells are EBNA positive (EBNA +). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 105 viable cells/mL. Maintain cell density between 3 X 105 and 3 X 106 viable cells/mL.xa0Do not allow the cell density to exceed 4 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 10,11 D5S818: 12 D7S820: 8,10 THO1: 7,9 TPOX: 6,8 vWA: 15,19 |
| Isotype： | kappa light chain |
| Name of Depositor： | NS Harris |
| Deposited As： | Homo sapiens |
| References： | Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735 . . J. Immunol. 118: 1480-1482, 1976. . . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973. Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 |