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| 产品名称： | pBJ-247 pBS-GAL1/10 |
|---|---|
| 商品货号： | TS214471 |
| Designations： | pBJ-247 pBS-GAL1/10 |
| Depositors： | C Hug |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7000000476837160 Vector: pBJ-247 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript II SK+ Marker(s):ampR Construct size (kb): 3.700000047683716 Features: marker(s): ampR promoter for expression: GAL10...GAL1 promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: BamHI...SacI MCS: KpnI...EcoRI |
| Applications： | contains easily purifiable cassette(s) for construction shuttle vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; SacI--3.7. The GAL10 RNA start site is disrupted, thus providing only one direction of transcription. Unless a start site for transcription is provided, little or no message should be made from inserts cloned into the GAL10 side of the promoter cassette. No pBS polylinker sites are included in the GAL1/GAL10 promoter cassette. Plasmid containing a BamHI-GAL1/GAL10-EcoRI cassette of 685 bp useful for making other constructions. The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - Kpn1/MCS/BamHI - GST-GAL1 promoter - GAL10 promoter - EcoRI/MCS/SacI - T3 promoter - lacI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Christopher Hug, personal communication |
| Shipped： | freeze-dried |