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| 产品名称： | pEFD53.2 |
|---|---|
| 商品货号： | TS214491 |
| Designations： | pEFD53.2 |
| Species： | Homo sapiens, human |
| Applications： | MspI also identifies a 2-allele RFLP (2.3, 1.9 kb) but this has not been tested by family studies. |
| Vector： | Construct size (kb): 8.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.699999809265137 Gene product: DNA Segment, single copy D1S73 Alleles: A1, A2 |
| Insert Size (kb)： | 4.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | An EcoRI/HindIII double digest reveals fragments of 2.5, 1.7, and 1.0 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.3, 2.6, 1.5, 0.86; HindIII--4.9, 3.4; EcoRI--7.4, 0.86; PstI--5.6, 1.9, 0.74; BamHI--5.8, 2.4; consistent with deposited DNA. Codominant segregation was demonstrated in 49 3-generation families. MspI also identifies a 2-allele RFLP (2.3, 1.9 kb) but this has not been tested by family studies. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI. |
| References： | Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 Fujimoto E, et al. Isolation and mapping of a polymorphic DNA sequence (pEFD53.2) on chromosome 1 D1S73. Nucleic Acids Res. 16: 3116, 1988. PubMed: 2897109 |