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| 产品名称： | MC2/3 |
|---|---|
| 商品货号： | TS214501 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Cell Type： | somatic cell hybrid |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | MC2/3 is a mouse chromosome 8 specific monochromosome hybrid cell line. The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains. The depositor has reported the presence of one copy of mouse chromosome 8 in each Chinese hamster cell by G band cytogenetics, fluorescence in situ hybridization (FISH) and PCR analysis. However, FISH analysis of ATCC frozen stocks by the depositor using biotinylated total genomic mouse DNA as probe indicates that 7 of 9 metaphases examined had a single mouse acrocentric chromosome present in a hamster background. An examination of interphase nuclei indicates that about 18% of the cells are tetraploid showing two distinct hybridization signals from the mouse while 82% are near diploid exhibiting one distinct signal. |
| Derivation： | The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains. Mouse chromosome 8 may be isolated with 96% purity. |
| Comments： | MC2/3 is a mouse chromosome 8 specific monochromosome hybrid cell line. This hybrid resulted from the fusion of adenine phosphoribosyl transferase negative (APRT -) Chinese hamster ovary cells with micronuclei from near euploid thymidine kinase negative (TK -) CAK mouse cells. Hybrids containing mouse chromosome 8 (which has the APRT locus) were selected in medium containing 0.075 mM adenine, 800 nM aminopterin and 0.016 mM thymidine (AAT). The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains. Mouse chromosome 8 may be isolated with 96% purity. The depositor has reported the presence of one copy of mouse chromosome 8 in each Chinese hamster cell by G band cytogenetics, fluorescence in situ hybridization (FISH) and PCR analysis. However, FISH analysis of ATCC frozen stocks by the depositor using biotinylated total genomic mouse DNA as probe indicates that 7 of 9 metaphases examined had a single mouse acrocentric chromosome present in a hamster background. Two of nine metaphases showed different rearrangements of the mouse acrocentric. An examination of interphase nuclei indicates that about 18% of the cells are tetraploid showing two distinct hybridization signals from the mouse while 82% are near diploid exhibiting one distinct signal. The level of tetraploidy may increase with additional passage. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium containing 0.075 mM adenine, 800 nM aminopterin, and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | RL Stallings |
| Deposited As： | Cricetulus griseus |
| Passage History： | The level of tetraploidy may increase with additional passage. |
| References： | Stallings RL, Deaven LL. A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation. Mamm. Genome 5: 572-573, 1994. PubMed: 8000142 |