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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS214534 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | P2PMC104 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | P2old+ mcrA delta(mcrBC-hsdRMS-mrr)102 supE supF |
| Type Strain： | no |
| Comments： | Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage A P2 lysogen of Escherichia coli PMC104 (ATCC 47061) High-efficiency, methylation-tolerant host for bacteriophage lambda with or without amber mutations. Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage. This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JP Doherty |
| References： | Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479 Judith P Doherty, personal communication |