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| 产品名称： | pRY121 plasmid in E. coli |
|---|---|
| 商品货号： | TS214559 |
| Designations： | pRY121 plasmid in E. coli |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | JM Becker |
| Vector： | Total size of construct (kb): 11.50 Markers: ampR, lacZ+, URA3 Excise insert: SalI/BamHI Vector: pLG67OZ Vector type: plasmid Vector size (kb): Markers: ampR |
| Insert： | Insert size (kb): 0.7 Gene: Galactose promoter region Source: Saccharomyces cerevisiae Insert ends: SalI/BamHI |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2(doublet), 2.8, 2.2; HindIII--6.2, 2.15, 1.45, 1.05; SalI--11.5. Contains S. cerevisiae GAL1 promoter region (from about 1 kb upstream of the GAL1 transcription start site to amino acid 33) fused to the bacterial lacZ gene. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | West RW Jr., et al. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol. Cell. Biol. 4: 2467-2478, 1984. PubMed: 6392852 |