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微信小章| 产品名称： | T24 |
|---|---|
| 商品货号： | TS214592 |
| Organism： | Homo sapiens, human |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | transitional cell carcinoma |
| Age： | 81 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers |
| Images： |  |
| Clinical Data： | Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. 81 years Caucasian female |
| Antigen Expression： | HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4 |
| Genes Expressed： | tumor specific antigen, HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4 |
| Cellular Products： | tumor specific antigen |
| Tumorigenic： | Yes |
| Effects： | Yes, in hamster cheek pouch No, in nude mice |
| Comments： | Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. Cells have a 19 hour generation time. Contains the ras (H-ras) oncogene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | C OToole |
| References： | OToole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: OToole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125. OToole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436 Williams BY, Schonbrunn A. Bombesin receptors in a human duodenal tumor cell line: binding properties and function. Cancer Res. 54: 818-824, 1994. PubMed: 8306345 Bubenik J, et al. Cellular and humoral immune responses to human urinary bladder carcinomas. Int. J. Cancer 5: 310-319, 1970. PubMed: 5452065 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Bubenik J, et al. Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int. J. Cancer 11: 765-773, 1973. PubMed: 4133950 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Bubenik J, et al. Cellular immunity to renal carcinomas in man. Int. J. Cancer 8: 503-513, 1971. PubMed: 5137312 Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 Ponton A, et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J. Biol. Chem. 271: 8991-8995, 1996. PubMed: 8621545 |