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| 产品名称： | pRLalpha2M/29J |
|---|---|
| 商品货号： | TS214642 |
| Designations： | pRLalpha2M/29J |
| GenBank Number： | J02635 |
| Species： | Rattus norvegicus, rat |
| Depositors： | GH Fey |
| Vector： | Construct size (kb): 7.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.599999904632568 Tissue: liver, male, acute phase Gene product: alpha-2-macroglobulin A2M |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The vector does not have convenient restriction sites close to the insert. A BamHI digest yields 3 fragments from the insert, 5 0.7, 3.54, 0.52 kb 3. The end fragments contain vector sequences, and the vector band is about 3 kb. The insert contains the following restriction sites (nucleotide position according to GenBank J02635): BglII--7; BamHI--576, 4116; PvuII--637, 2174, 2536, 3040; HindIII--1440; XbaI--2217. Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 3.0, 0.6, 0.5; EcoRI--4.6, 2.6, 0.4; PstI--4.3, 2.0, 1.3; HindIII--5.6, 2.0; SalI--7.6. Contains the complete coding sequence (nt 64-4482). |
| References： | Gehring MR, et al. Sequence of rat liver alpha2-macroglobulin and acute phase control of its messenger RNA. J. Biol. Chem. 262: 446-454, 1987. PubMed: 2432068 Georg H Fey, personal communication |