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| 产品名称： | p413 GPD |
|---|---|
| 商品货号： | TS214645 |
| Designations： | p413 GPD |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Intact vector size: 5.786
Type of vector: plasmid Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS413 Host range: Saccharomyces cerevisiae, Candida robusta; Escherichia coli Features (with orientation and position when available): marker(s): HIS3, →, 200-1395 terminator: CYC1, →, 2077-2337 MCS: SalI...XbaI, →, 2343-2401 promoter for expression: GPD, ←, 2401-3081 marker(s): ampR, ←, 4328-5258 replicon: CEN6/ARSH4, →, 5363-5876 Vector: p413 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS413
Marker(s): ampR, HIS3
Construct size (kb): 5.851
Features: marker(s): HIS3 marker(s): ampR promoter for expression: GPD replicon: CEN6/ARSH4 MCS: SalI...XbaI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.7; EcoRI--5.9; BamHI--5.9. Low copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | Component of: ATCC 87669 |
| Shipped： | frozen |