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| 产品名称： | S194/5.XXO-1 |
|---|---|
| 商品货号： | TS214646 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | This line was derived from an IgA secreting mineral oil induced BALB/c myeloma. Both heavy and light chains are synthesized but not secreted. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This line was derived from an IgA secreting mineral oil induced BALB/c myeloma. |
| Antigen Expression： | H-2d; PC.1 |
| Genes Expressed： | immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; not secreted |
| Comments： | This line was derived from an IgA secreting mineral oil induced BALB/c myeloma. Both heavy and light chains are synthesized but not secreted. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgA (not secreted); kappa light chain (not secreted) |
| Name of Depositor： | R Hyman, P Ralph, S Sarkar |
| Deposited As： | Mus musculus |
| References： | Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789 |