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| 产品名称： | pCMVlacZII |
|---|---|
| 商品货号： | TS214719 |
| Designations： | pCMVlacZII |
| Depositors： | LA Culp |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 10.8999996185302700 DESCRIPTION OF VECTOR: Intact vector size: 10.900 Type of vector: plasmid Cloning sites: Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): neoR promoter: CMV, RSV replicon: pMB1, M13, SV40, polyoma terminator: SV40 Vector: pCMVlacZII (plasmid) Promoters: Promoter CMV Marker(s):neoR Construct size (kb): 10.89999961853027 Features: marker(s): neoR promoter: CMV, RSV replicon: pMB1, M13, SV40, polyoma terminator: SV40 |
| Applications： | reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0. Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is: CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori. |
| Media： | ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jo H, et al. Differential effect of shear stress on extracellular signal-regulated kinase and N-terminal Jun kinase in endothelial cells. Gi2- and Gbeta/gamma-dependent signaling pathways. J. Biol. Chem. 272: 1395-1401, 1997. PubMed: 8995450 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |