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| 产品名称： | p171 |
|---|---|
| 商品货号： | TS214721 |
| Designations： | p171 |
| GenBank Number： | K01691 |
| Species： | Moloney murine sarcoma virus, Moloney murine sarcoma virus |
| Depositors： | UA Rapp |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 0.699999988079071 Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) RAF1 Alleles: B, M, B1, A1, A2, B2 |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--4.38; EcoRI/PvuII--2.3, 1.8; NruI--4.2; EcoRI/NruI--3.4, 0.84. The insert contains AvaI, AvaII, StuI(2), HpaI, and SphI sites. Constructed by inserting an XhoI/SstII fragment into vector from which the HindIII/SalI fragment had been removed. The SstIII and HindIII sites were blunt-ended before ligation. |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| References： | Mark GE, Rapp UR. Primary structure of v-raf: relatedness to the src family of oncogenes. Science 224: 285-289, 1984. PubMed: 6324342 Rapp UR, et al. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Natl. Acad. Sci. USA 80: 4218-4222, 1983. PubMed: 6308607 Kozak C, et al. A new oncogene, c-raf, is located on mouse chromosome 6. J. Virol. 49: 297-299, 1984. PubMed: 6690719 |