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| 产品名称： | YEp368R |
|---|---|
| 商品货号： | TS214727 |
| Designations： | YEp368R |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3999996185302730 Vector: YEp368R (plasmid) Promoters: Promoter none Construction: YEp363A, YEp358R (ATCC 37739) Marker(s):LEU2,ampR Construct size (kb): 8.399999618530273 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: none replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The EcoRI, KpnI, and SacI sites are not unique. Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; PstI--8.4. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37740 - 37742) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3, from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |