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| 产品名称： | pG60/T10 |
|---|---|
| 商品货号： | TS214729 |
| Designations： | pG60/T10 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 18.5 |
| Insert： | DNA: genomic Insert lengths(kb): 6.699999809265137 Gene product: 5 end processing factor CBP1 |
| Insert Size (kb)： | 6.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximate): BamHI- 0.8- BamHI- 0.6- BamHI- 0.7- HindIII- 2.8- BamHI. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--7.8, 4.0, 3.5, 2.3, 0.9; XhoI--11.6, 7.0; XbaI--18.5; XbaI/AvaI--7.0, 3.5, 2.4, 2.0, 1.7, 0.6; BamHI--16.0, 2.5. The insert includes a continuous open reading frame encoding a basic protein of 654 amino acids and 76 kDa. The mRNA is about 2.2 kb. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Dieckmann CL, et al. Assembly of the mitochondrial membrane system. Nucleotide sequence of a yeast nuclear gene (CBP1) involved in 5 end processing of cytochrome b pre-mRNA. J. Biol. Chem. 259: 4732-4738, 1984. PubMed: 6325407 |