微信小陈
微信小章| 产品名称： | TF-1a |
|---|---|
| 商品货号： | TS214733 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | erythroblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | erythroleukemia |
| Age： | 35 years |
| Gender： | male |
| Ethnicity： | Japanese |
| Applications： | It can be used to study signal pathways involved in the spontaneous and factor-induced growth of the cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | TF-1a is a factor-independent variant isolated from the factor-dependent TF-1 cell line (ATCC CRL-2003). |
| Clinical Data： | 35 years Japanese male |
| Antigen Expression： | CD34+, CD38- |
| Tumorigenic： | Yes |
| Effects： | Yes, in soft agar Yes, the cells are tumorigenic in nude mice |
| Comments： | TF-1a is a factor-independent variant isolated from the factor-dependent TF-1 cell line (see ATCC CRL-2003).
The cells retain the ability to respond to a variety of cytokines, with a different response pattern from the parental cell line. TF-1a, but not TF-1 cells, form colonies in soft agar culture in the absence of any added growth factors, and generate invasive tumors in nude mice. There is a slight constitutive activation of the MAP kinase and MEK proteins in TF-1a but not in TF-1 cells. Phenotypically, TF-1 cells are CD34 positive and CD38 positive, whereas TF-1a cells are CD34 positive and CD38 negative. TF1-a cells, but not TF-1 cells, are resistant to tumor necrosis factor alpha (TNF-alpha) induced apoptosis. TF-1a is a model for studying human primitive myeloid progenitor cells and for studying the process of progressive malignant transformation of myeloid cells.
It can be used to study signal pathways involved in the spontaneous and factor-induced growth of the cells. A culture submitted to the ATCC in April of 1999 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 105 viable cells/mL. Maintain cell density between 3 x 105 and 3 x 106 viable cells/mL. Medium Renewal:xa02 to 3 times a week.xa0 |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 13 D13S317: 8,9 D16S539: 9,12 D5S818: 13 D7S820: 12 THO1: 7,9 TPOX: 8 vWA: 15,17 |
| Name of Depositor： | X Hu |
| Deposited As： | human |
| References： | Hu X, et al. Characterization of a unique factor-independent variant derived from human factor-dependent TF-1 cells: a transformed event. Leuk. Res. 22: 817-826, 1998. PubMed: 9716013 |