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微信小章| 产品名称： | B6H12.2 |
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| 商品货号： | TS214750 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors). Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against integrin associated protein (CD47) and vitonectin receptor (VnR) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against integrin associated protein (CD47) and vitonectin receptor (VnR) |
| Comments： | The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD). The antibody also inhibits RGD stimulated phagocytosis. It precipitates a heterodimer of apparent molecular weights 140000 and 105000 daltons. The antibody does not react with gpIIb/III or the LFA-1/Mac-1 family of cellular adhesion receptors. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 80%; fetal bovine serum, 20%
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105xa0viable cells/mL. Maintain cultures at cell concentrations between 2 x 105 and 1 x 106xa0viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°Cxa0 |
| Isotype： | IgG1 |
| Name of Depositor： | Washington Univ. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Brown EJ. Novel monoclonal antibodies. US Patent 5,057,604 dated Oct 15 1991 Lindberg FP, et al. Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J. Biol. Chem. 269: 1567-1570, 1994. PubMed: 8294396 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |