微信小陈
微信小章| 产品名称： | S16Y |
|---|---|
| 商品货号： | TS214756 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | sciatic nerve |
| Cell Type： | Schwann cell |
| Product Format： | frozen |
| Morphology： | spindle-shaped |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | neonatal |
| Applications： | 16Y, S16 (ATCC CRL-2941) and S42 (ATCC CRL-2942) cells should be useful for investigating the cell biology of MAG and other myelin-related components as the levels of MAG expression in the three lines are inversely related to their rates of proliferation. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The S16Y cell line arose spontaneousely from a passage of the S16 (ATCC CRL-2941), a line that was derived from a primary culture of Schwann cells and was immortalized by repetitive passaging. |
| Antigen Expression： | Myelin-associated glycoprotein (MAG), not expressed Galactocerebroside (GalC), not expressed P0 glycoprotein, not expressed |
| Genes Expressed： | Myelin-associated glycoprotein (MAG), not expressed,Galactocerebroside (GalC), not expressed,P0 glycoprotein, not expressed |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: The culture flasks should be treated with 0.1mL/cm2xa0of flask surface area with 15 µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.xa0
Subcultivation ratio: A subcultivation ratio of 1:5 to 1:12 twice weekly is recommended. Medium renewal: Every 2 to 3 days. |
| Cryopreservation： | Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Population Doubling Time： | approximately 20 hours |
| Name of Depositor： | RH Quarles |
| Year of Origin： | 1989 |
| References： | Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597 Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958 Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295 |