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| 产品名称： | pAB520 pAmPh520 |
|---|---|
| 商品货号： | TS214759 |
| Designations： | pAB520 pAmPh520 |
| Depositors： | BM Tyler |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0999999046325680 Vector: pAB520 (plasmid) Construction: pAB366, pUC18 Marker(s):ampR,bleR Construct size (kb): 4.099999904632568 Features: marker(s): ampR, bleR replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.4; KpnI--4.1; SmaI--3.1, 1.0. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Shuttle vector conferring bleomycin and phleomycin resistance. That expression was improved by modifying the 17 bp before the initiation codon in pAB366 to match the sequence of the am gene. The am::ble fusion cassette can be removed by digestion with either HindIII, SphI, XbaI or BamHI or any combination of them. The order of the major features in this plasmid is: pUC18 - MCS (HindIII to BamHI) - am promoter - ble - XbaI - am 3 region - EcoRI - MCS (HindIII to EcoRI) - pUC18. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844 |
| Shipped： | freeze-dried |