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| 产品名称： | pCaMV-CB3 |
|---|---|
| 商品货号： | TS214787 |
| Designations： | pCaMV-CB3 |
| Species： | Cauliflower mosaic virus |
| Depositors： | JE Schoelz |
| Applications： | This isolate produces milder symptoms in turnips than the strain cloned in ATCC 45145 does. |
| Vector： | Construct size (kb): 10.70199966430664 |
| Insert： | DNA: cDNA Insert lengths(kb): 8.031000137329102 |
| Insert Size (kb)： | 8.031 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.3, 4.2, 0.25; EcoRI--4.3, 3.0, 2.6, 0.55, 0.2; SacI--8.0, 2.8. The insert contains the following restriction sites (according to genomic position): XhoI--1642; BamHI--1926, 2148; EcoRI--2414, 2474, 3034, 5646, 6105; BglI--3408. The clone is infectious. This isolate produces milder symptoms in turnips than the strain cloned in ATCC 45145 does. This isolate also infects Nicotiana bigelovii and N. clevelandii. Contains the compete genome, interrupted at the SacI site that occurs at nt 5822. |
| Classification： | Caulimovirus |
| References： | James E Schoelz, personal communication |