宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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CHED

货号 TS214794
中文名称 null
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产品名称: CHED
商品货号: TS214794
Designations: CHED
GenBank Number:

M80629

Species: Homo sapiens, human
Depositors: H Soreq
Vector:
Construct size (kb): 5.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBS(+)
Intact vector size: 3.204
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI HincII AccI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI HincII AccI PstI SphI
HindIII
Construction: pUC19, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CHED*
Gene name: cholinesterase-related cell division controller
Contains complete coding sequence?: Y
Tissue: glioblastoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: M80629
Insert lengths(kb): 2.200000047683716
Tissue: glioblastoma
Gene product: cholinesterase-related cell division controller CHED*
Insert Size (kb): 2.200
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BglII--3.5, 1.75; EcoRI--3.1, 2.2; HindIII--3.35, 1.8; PstI--3.3, 1.4, 0.46, 0.1; XbaI--5.2.
The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.19, 2.0; PstI--0.35, 1.73, 1.83; HindIII--0.39; BamHI--1.88.
Contains the complete coding sequence (nt 772-2028).
Detects transcripts of 2.2-2.3 kb.
References:

Lapidot-Lifson Y, et al. Cloning and antisense oligodeoxynucleotide inhibition of a human homolog of cdc2 required in hematopoiesis. Proc. Natl. Acad. Sci. USA 89: 579-583, 1992. PubMed: 1731328