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| 产品名称： | Clone 9 UTR-02817, WI-7764 |
|---|---|
| 商品货号： | TS214796 |
| Designations： | Clone 9 UTR-02817, WI-7764 |
| GenBank Number： | M58603 |
| Species： | Homo sapiens, human |
| Depositors： | C Scheidereit, Max Planck Institut fur Molekulare Genetik |
| Vector： | Name of construct: clone 9 Other name(s): WI-7764; UTR-02817 Excise insert: EcoRI Size of construct (kb): 6.8 Markers: ampR Name of vector: pBluescript II SK Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, T7, T3 replicon: pMB1, f1 |
| Insert： | Genome: Homo sapiens Gene symbol: NFKB1 Genomic copy number: unique Gene name: nuclear factor of kappa light chain gene enhancer in B-cells 1 (p105) Contains complete coding sequence?: Y Chromosome: 4; Localization: 4 q24; 4, 538.149999 cR Tissue: BJA-B cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 3.821 Cross references: DNA Seq. Acc.: M58603 |
| Insert Size (kb)： | 3.821 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | ATCC 33694 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9. Restriction digests of the clone give the following sizes (kb): PstI--4.0, 1.9, 1.0; BglI--3.0, 2.4, 1.2, 0.3; EcoRI--3.9, 3.0; SalI--6.9. The insert includes the following restriction sites (nt from the 5 end): PvuII--958, 1172, 2576, 2739, 2827, 2849, 3572, 3589; XbaI--1900, 2722, 3286; BamHI--448; SmaI--284; HindIII--3563. Contains the complete coding sequence, plus 5 (397 bp) and 3 (520 bp, including the stop codon) untranslated regions. Extends 196 bp beyond the reported sequence in the 3 direction. |
| References： | Meyer R, et al. Cloning of the DNA-binding subunit of human nuclear factor kappaB: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha. Proc. Natl. Acad. Sci. USA 88: 966-970, 1991. PubMed: 1992489 Claus Scheidereit, personal communication |