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| 产品名称： | p426 GPD |
|---|---|
| 商品货号： | TS214817 |
| Designations： | p426 GPD |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.6370000839233400
DESCRIPTION OF VECTOR:
Intact vector size: 6.637 Type of vector: plasmid Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS426 (ATCC 77107) Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): Marker(s): URA3, ->, 200-1300 Terminator: CYC1, ->, 2004-2264 MCS: XhoI...XbaI, ->, 2265-2339 Promoter for expression: GPD, <-, 2339-3019 Marker(s): ampR, <-, 4138-5068 Replicon: 2 micron, ->, 5167-6507 Vector: p426 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS426 (ATCC 77107)
Marker(s):xa0URA3,ampR
Construct size (kb): 6.63700008392334
Features: marker(s): URA3 Marker(s): ampR Promoter for expression: GPD Replicon: 2 micron MCS: XhoI...XbaI Terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.7; EcoRI--6.6; BamHI--6.6. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | frozen |