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| 产品名称： | pro-caspase-3 |
|---|---|
| 商品货号： | TS214845 |
| Designations： | pro-caspase-3 |
| GenBank Number： | U26943 |
| Species： | Homo sapiens, human |
| Depositors： | G Salvesen |
| Applications： | in another host, produces protein apopain in another host, produces protein caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32 |
| Vector： | Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pET-23b Intact vector size: 3.665 Type of vector: plasmid Vector end: NdeI Vector end: NdeI Cloning sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI Polylinker sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI Other unique sites: NheI NdeI XbaI Host range: Escherichia coli Features (with orientation and position when available): transcription terminator: T7, epitope tag: hexahistidine sequence (6-His), MCS: BamHI...XhoI, epitope tag: T7 tag sequence, promoter for in vitro transcription: T7, replicon: pMB1, 1450-1450 marker(s): ampR, replicon: f1, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CASP3 Other gene symbols: CPP32; CPP32B; Yama; apopain Gene name: caspase 3, apoptosis-related cysteine protease Contains complete coding sequence?: Y Chromosome: 4; Localization: 4 q34 Tissue: umbilical vein endothelial cells Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.9 Cross references: DNA Seq. Acc.: U26943 Nucleotides ~1-900 of the insert correspond to nucleotides ~1-834 of U26943. Insert lengths(kb): 0.8999999761581421 Tissue: umbilical vein endothelial cells Gene product: caspase 3, apoptosis-related cysteine protease( Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32) Yama Target Gene: apopain, caspase 3, apoptosis-related cysteine protease |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NdeI--4.5; XbaI/XhoI--4.0, 0.95; HindIII--4.5. Plasmid encodes the complete coding sequence, tagged at the C-terminus with hexahistidine. |
| References： | Orth K, et al. Molecular ordering of apoptotic mammalian CED-3/ICE-like proteases. J. Biol. Chem. 271: 20977-20980, 1996. PubMed: 8702858 Quan LT, et al. Proteolytic activation of the cell death protease Yama/CPP32 by granzyme B. Proc. Natl. Acad. Sci. USA 93: 1972-1976, 1996. PubMed: 8700869 Stennicke HR, Salvesen GS. Biochemical characteristics of caspases-3, -6, -7, and -8. J. Biol. Chem. 272: 25719-25723, 1997. PubMed: 9325297 |