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| 产品名称： | pRSVcat |
|---|---|
| 商品货号： | TS214873 |
| Designations： | pRSVcat |
| Depositors： | BH Howard |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1 Vector: pRSVcat (plasmid) Promoters: Promoter RSV LTR Construction: pSV2-betaG, Tn9, RSV Marker(s):ampR,cmlR Construct size (kb): 5.1 Features: marker(s): ampR, cmlR promoter: RSV LTR replicon: pMB1 enhancer: RSV |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--5.1; BamHI--5.1; PstI--4.1, 1.0. Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BglII--uncut; BamHI--5.0. Vector for expression of chloramphenicol acetyltransferase in eukaryotic cell lines under the control of Rous Sarcoma Virus long terminal repeats. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240 Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651 Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze-dried Escherichia coli HB101 containing the plasmid. |