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| 产品名称： | pGC24 plasmid in E. coli |
|---|---|
| 商品货号： | TS214883 |
| Designations： | pGC24 plasmid in E. coli |
| Species： | Aspergillus awamori Nakazawa |
| Depositors： | Cetus Corp., A Belt, Cetus Corp. |
| Applications： | produces protein glucoamylase |
| Vector： | Construct size (kb): 6.55 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTRP3 Intact vector size: 4.460 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: HindIII Polylinker sites: Other unique sites: BamHI Construction: pVH153, pBR322 Host range: Escherichia coli Features (with orientation and position when available): promoter: trp, -> ribosome-binding site: , -> restriction site: HindIII, -> marker(s): tetR, -> replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Aspergillus awamori Gene name: glucoamylase Contains complete coding sequence?: U Strain: NRRL 15271 Type of DNA: cDNA Insert end: HindIII Insert end: HindIII Insert size (kb): 2.11 Cross references: Insert lengths(kb): 2.10 Gene product: glucoamylase Target Gene: glucoamylase |
| Insert Size (kb)： | 2.110 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains internal PstI and BamHI sites. Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.4; PstI--5.2, 1.9; BamHI--5.8, 1.1. Expresses the gene product under the control of the Escherichia coli trp promoter. The vector was constructed by inserting an EcoRI/ClaI fragment with Escherichia coli trp promoter and RBS into pBR322. A HindIII fragment of about 2.2 kb containing the gene with poly(A) signal was cloned into the HindIII site. |
| References： | Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |