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| 产品名称： | BMEC-1 |
|---|---|
| 商品货号： | TS214908 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Studies in Cancer, tumor metastasis, endothelial function, cell trafficking, surface molecule interactions, etc. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Comments： | This cell line was infected with SV-40 Large T antigen. The depositor stated that the cell line has passage limitation ≤ 27. Therefore, harvest prior to passage 27. |
| Complete Growth Medium： | The base medium for this cell line is MCDB-131 (Gibco cat # 10372-019). To make the complete medium add to 500 mL of the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Interval: Maintain cultures at a cell concentration between 1.0 x 104 and 3.0 x 104 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth media 95% + 5% DMSO (ATCC 4-X) |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% CO2
Temperature: 37°C |
| Cells per Vial： | Approximately 2.0 to 3.0 x 106 cells |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,10
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 8,9
vWA: 14,17 |
| Sterility Tests： | Bacteria and yeast: No growth Mycoplasma: No growth |
| Viability： | ≥ 50% |
| Name of Depositor： | J Moon, National Center for Emerging and Zoonotic Infectious Diseases, CDC |
| Year of Origin： | 1995 |
| References： | Candal FJ, et al. BMEC-1: a human bone marrow microvascular endothelial cell line with primary cell characteristics. Microvasc Res 52(3):221-34, 1996. PubMed: 8954864 |