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微信小章| 产品名称： | Pseudomonas aeruginosa (Schroeter) Migula |
|---|---|
| 商品货号： | TS215040 |
| Verified By： | Whole-genome Sequencing |
| Strain Designations： | PAO1-LAC |
| Application： | Transformation host Opportunistic pathogen research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | lacIq+ delta(lacZ)M15+ tetA+ tetR+ |
| Genome Sequenced Strain： | Pseudomonas aeruginosa PAO1, complete genome |
| Type Strain： | no |
| Comments： | For use with the expression vector pUCP19 (ATCC 87110).
Phenotype verified: tetR.
Constructed by stable integration of a mini-D3112 transposable element containing sequences allowing lacZalpha complementation.
Purified genomic DNA from this organism is available as TS215040D-5.
Genome sequenced strain |
| Medium： | ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30°C Atmosphere: Aerobic |
| Name of Depositor： | RR Karkhoff-Schweizer |
| Special Collection： | NCRR Contract |
| References： | Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342 Stover CK, et al. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406: 959-964, 2000. PubMed: 10984043 |
| Cross References： | Nucleotide (GenBank) : AE004091 Pseudomonas aeruginosa PAO1, complete genome |