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| 产品名称： | MEG-01 |
|---|---|
| 商品货号： | TS215171 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | megakaryoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | chronic myelogenous leukemia (CML) |
| Age： | 55 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present |
| Images： |  |
| Derivation： | The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML. |
| Clinical Data： | male
55 years |
| Antigen Expression： | CD41 +; CD61 +; CDw14 + |
| Comments： | The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.
They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, platelet).
They are negative for other lymphoid and myeloid seris antigens. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 105 cells/mL into fresh medium in a new flasks. Keep culture below approximate density of 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 8 D16S539: 9 D5S818: 13 D7S820: 11 THO1: 7 TPOX: 8,11 vWA: 16 |
| Population Doubling Time： | 36 to 48 hrs |
| Name of Depositor： | M Tekeuchi, MJ Fitzgerald |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1983 |
| References： | Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511 |