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| 产品名称： | CMT-93 |
|---|---|
| 商品货号： | TS215173 |
| Organism： | Mus musculus, mouse |
| Tissue： | rectum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | polyploid carcinoma |
| Age： | 19 months |
| Gender： | male |
| Strain： | C57BL/icrf |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | male |
| Antigen Expression： | H-2b |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
|
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | LM Franks |
| Deposited As： | Mus musculus |
| References： | Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371 |