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| 产品名称： | HCM-BROD-0106-C71 |
|---|---|
| 商品货号： | TS215181 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Product Format： | frozen 1.0 mL |
| Morphology： | neurosphere |
| Culture Properties： | aggregate/suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Recurrent glioblastoma |
| Age： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71 |
| Gender： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71 |
| Ethnicity： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71 |
| Applications： | Basic research, compound screening. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | ICD-10-CM code: C71, malignant neoplasm of cerebrum See associated clinical data for patient profile information, if available.https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71 |
| Comments： | Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data. https://ocg.cancer.gov/programs/hcmi/resources https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71 |
| Complete Growth Medium： | NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980). Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751 |
| Subculturing： | Important: use Ultra Low Attachment (ULA) flasks/plates when culturing this model such as Corning #3814.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5-5 x 105 cells/mL and maintain between below 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). Large neurospheres should be mechanically dissociated to small fragments by repeated pipetting. Do not dissociate to single cells. |
| Cryopreservation： | Complete growth media containing 10% DMSO (ATCC 4-X) |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial： | ≥ 1 x 106 cells |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9
D16S539: 9,13
D5S818: 12,13
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 15,16 |
| Sterility Tests： | Bacteria, yeast and fungi: No growth Mycoplasma: No growth |
| Viral Testing： | Hepatitis B: None detected Cytomegalovirus: None detected Human immunodeficiency virus: None detected Epstein-Barr virus: None detected Human papillomavirus: None detected |
| Name of Depositor： | Broad Institute |
| Year of Origin： | 2018 |