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微信小章| 产品名称： | HCM-SANG-0270-C20 |
|---|---|
| 商品货号： | TS215183 |
| Organism： | Homo sapiens, human |
| Tissue： | rectum |
| Product Format： | frozen 1.0 mL |
| Morphology： | organoid |
| Culture Properties： | embedded in 3D culture |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Primary Adenocarcinoma |
| Age： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-SANG-0270-C20 |
| Gender： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-SANG-0270-C20 |
| Ethnicity： | See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-SANG-0270-C20 |
| Applications： | Research purposes, compound screening |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | ICD-10-CM code: C20, Malignant neoplasm of rectum See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-SANG-0270-C20 |
| Comments： | Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data. https://ocg.cancer.gov/programs/hcmi/resources https://portal.gdc.cancer.gov/xa0xa0 https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-SANG-0270-C20 |
| Complete Growth Medium： | To prepare the complete medium for this organoid model please refer to the Organoid Media Formulation #4. |
| Subculturing： | Initiating culture from frozen vials:xa0For a brief overview of the thawing procedure see our quickstart guidexa0Thawing Cryopreserved Human Organoids. Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well.xa0 Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture.xa0 For a brief overview of the subculture and expansion of organoids see our quickstart guidexa0Subculture and Expansion of Human Organoids Protocol. For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology |
| Cryopreservation： | We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-3020). For a brief overview of the cryopreservation procedure for organoids see our quickstart guidexa0Organoid Cryopreservation Protocol. For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology. |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial： | ≥ 1 x 106 |
| Volume： | 1.0 mL |
| Sterility Tests： | Bacteria and yeast: No growth Mycoplasma: No growth |
| Viral Testing： | Hepatitis B: None detected Cytomegalovirus: None detected Human immunodeficiency virus: None detected Epstein-Barr virus: None detected Human papillomavirus: None detected |
| Name of Depositor： | Wellcome Sanger Institute |
| Year of Origin： | 2017 |