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| 产品名称： | LASCPC-01 |
|---|---|
| 商品货号： | TS215202 |
| Organism： | Homo sapiens, human |
| Tissue： | prostate |
| Product Format： | frozen 1.0 mL |
| Morphology： | aggregate |
| Culture Properties： | suspension/aggregate |
| Biosafety Level： | 2 xa0Cells contain lentivirus genes
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | prostate cancer |
| Age： | 75-80 |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC) |
| Karyotype： | Conventional karyotyping: normal 46 X,Y Array CGH: mosaic gian of 1q23.1-1q44 |
| Images： |  |
| Clinical Data： | 75-80 years male Caucasian |
| Comments： | Expression of RFP, GFP, MYCN, and myristoylated AKT1. |
| Complete Growth Medium： | To prepare 500mL of complete medium:
Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added. |
| Subculturing： | Cultures can be maintained by addition of fresh medium once or twice a week. |
| Cryopreservation： | liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial： | Since being aggregates we use 20 ml culture medium with healthy aggregates per 1 ml freeze volume |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 9
TPOX: 8,11
vWA: 15,17 |
| Sterility Tests： | Bacteria and yeast:xa0No growthxa0 Mycoplasma:xa0No growth |
| Viability： | ≥ 50% |
| Population Doubling Time： | Approximately 24 hours per depositor |
| Name of Depositor： | J Lee |
| Year of Origin： | 2014 |
| References： | Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099 |