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| 产品名称： | pRS424 |
|---|---|
| 商品货号： | TS215203 |
| Designations： | pRS424 |
| Depositors： | TW Christianson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.616
Vector: pRS424 (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pJS92, pRS304, pBluescript II SK+
Marker(s):TRP1,ampR
Construct size (kb): 5.616000175476074
Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: T3 promoter: T7 (phi10) promoter: lac replicon: 2 micron replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; PvuII--5.2, 0.5; XbaI--3.3, 1.4, 1.0. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 1.5 + or - 1.7% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - TRP1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568 |
| Related Products： | component of:ATCC 77108 component of:ATCC 87532 component of:ATCC 87535 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli containing the phagemid. |