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微信小章| 产品名称： | LNCaP clone FGC-Luc2 |
|---|---|
| 商品货号： | TS215233 |
| Organism： | Homo sapiens, human |
| Tissue： | prostate; derived from metastatic site: left supraclavicular lymph node |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent, single cells and loosely attached clusters |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 50 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drug. It also can be used in cell-based assays for cancer research. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Tumorigenic： | Yes, tested in NSG mice |
| Comments： | This luciferase expressing cell line was derived from parental line CRL-1740 by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivoxa0bioluminescence assays. The cells should be maintained in blasticidin (2 µg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Interval: Maintain cultures at a cell concentration between 2 X 104 and 1.0 X 105
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial： | ≥ 1.0 x 106 cells |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 10,12
D16S539: 11
D5S818: 11,12
D7S820: 9.1,10.3
THO1: 9
TPOX: 8,9
vWA: 16,18 |
| Sterility Tests： | Bacteria and yeast: No growth Mycoplasma: No growth |
| Viral Testing： | Hepatitis B: None detected Cytomegalovirus: None detected Human immunodeficiency virus: None detected Epstein-Barr virus: None detected Human papillomavirus: None detected |
| Functional Tests： | Luciferase activity: signal to noise ≥ 1,000 RLUs In Vitro Luminesence: 100,000 photons/cell/sec, subject to imaging and culturing conditions |
| Population Doubling Time： | approximately 41 hrs |
| Name of Depositor： | ATCC |
| Year of Origin： | 2018 |
| References： | Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337 Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638 Models for prostate cancer. 37New York: Liss; 1980. Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201 Horoszewicz JS, et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. PubMed: 6831420 Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: 8662737 |