微信小陈
微信小章| 产品名称： | BCE C/D-1b |
|---|---|
| 商品货号： | TS215235 |
| Organism： | Bos taurus, cow |
| Tissue： | eye, cornea |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | male |
| Applications： | The cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This line was established from explants of normal adult bovine corneas. |
| Genes Expressed： | extracellular matrix (ECM) proteins |
| Cellular Products： | extracellular matrix (ECM) proteins |
| Comments： | The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90. The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, add fresh trypsin (0.25%) - EDTA (0.03%) solution, rinse and remove. Add fresh trypsin solution (1 to 2 mL) and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: 1:2 to 1:4
Medium Renewal: 2 to 3 times per week
|
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | AH Nicholas, C Bethea |
| Deposited As： | Bos taurus |
| Passage History： | The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90. |
| References： | Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215 Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171 |