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| 产品名称： | HCE-2 50.B1 |
|---|---|
| 商品货号： | TS215237 |
| Organism： | Homo sapiens, human |
| Tissue： | eye, cornea |
| Cell Type： | epithelial Adenovirus 12-SV40 hybrid transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Adeno-12/SV-40 viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | aneuploid; Y chromosome is present |
| Images： |  |
| Derivation： | A primary culture of normal corneal epithelium was immortalized with Ad12-SV40 hybrid virus by overnight incubation with virus at 37°C |
| Clinical Data： | Black male |
| Effects： | Yes, in soft agar |
| Comments： | Keratinocyte Serum-Free medium is available from GIBCO, Grand Island, New York. |
| Complete Growth Medium： | Keratinocyte-Serum Free Medium (Gibco 17005-042) Supplemented with frozen additives included (from GIBCO): 1) 0.05 mg/ml bovine pituitary extract (BPE) 2) 5 ng/ml epidermal growth factor (EGF). NOTE: Do not filter EGF And also supplemented with 500 ng/ml hydrocortisone and 0.005mg/ml insulin (not included). |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: 85% Growth Medium, 10% FBS, 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 8,11 D16S539: 9,11 D5S818: 11,12 D7S820: 8,10 THO1: 6,9 TPOX: 10,11 vWA: 15,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, A GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2 |
| Population Doubling Time： | 30 hrs |
| Name of Depositor： | CR Kahn, Gillette Medical Evaluation Laboratories |
| U.S. Patent Number： | |
| References： | Kahn CR, Rhim J. Human corneal epithelial cell lines with extended lifespan. US Patent 5,585,265 dated Dec 17 1996 Walker TL, Kahn CR. Human corneal epithelial cell lines with extended lifespan. US Patent 5,672,498 dated Sep 30 1997 |