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| 产品名称： | NG108-15 108CC15 |
|---|---|
| 商品货号： | TS215250 |
| Organism： | Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma) |
| Tissue： | brain |
| Cell Type： | somatic cell hybrid |
| Product Format： | frozen |
| Morphology： | flat; round; 10 to 100 micrometers diameter |
| Culture Properties： | Adherent, but please note: as the culture media becomes acidic these cells begin to detach and grow as a suspension, but they will typically reattach again when fresh medium is added. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | glioblastoma; neuroblastoma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： | 
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| Derivation： | The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. |
| Complete Growth Medium： | The base medium for this cell line is Dulbeccos Modified Eagles Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | Univ. Texas Southwestern Medical Cntr. |
| Deposited As： | mouse (neuroblastoma); rat (glioma) |
| U.S. Patent Number： | |
| References： | Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829 Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920 Bernd Hamprecht, personal communication |