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Polynucleobacter acidiphobus

货号 TS244486
中文名称 null
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产品名称: Polynucleobacter acidiphobus
商品货号: TS244486
<strong>Name </strong>: Polynucleobacter acidiphobus Hahn et al. 2011
<strong>DSM No. </strong>: 21994, Type strain dsm-21994 dsm21994 dsm 21994 Polynucleobacter acidiphobus
Strain designation : MWH-PoolGreenA3
Other collection no. <br />or WDCM no. : CIP 110079
Isolated from : freshwater from a rock pool
Country : France
Corsica, 42° 10 21.05\ N 8° 53 54.54\E
Date of sampling : 23.06.2005
<strong>Nagoya Protocol Restrictions </strong>: There are NO known Nagoya Protocol restrictions for this strain.
History :
Genbank accession numbers : 16S rRNA gene: FM208180
Cultivation conditions : Medium 830 dilute R2A medium 1:5 and add Na-pyruvate and cysteine-HCl x H2O, 2 g per L each, 25°C
or
Medium 1167 , agar surface must be kept moist all the time, 25°C
Medium/media recipe(s) required for preparing 1167:
- xa0Medium 14
- xa0Medium 27

Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.

Incubation time: 3-7 days

Complete DSMZ Media List
Summary and <br />additional information : Type strain. Taxonomy/description (15908). Sequence accession no. 16S rRNA gene: FM208180. (Medium 830, dilute R2A medium 1:5 and add Na-pyruvate and cysteine-HCl x H2O, 2 g per L each, or Medium 1167, 25°C, agar surface must be kept moist all the time). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
For detailed information : BacDive - The Bacterial Diversity Metadatabase
Literature : 15908
Risk group : 1 (classification according to German TRBA)
Other cultures : All DSMZ cultures of the species