微信小李
微信小章
产品简介
购买须知
| 产品名称： | L-929 |
|---|---|
| 商品货号： | TS258762 |
| <strong>Cell line </strong>： | L-929 |
| DSMZ no. ： | TS258762 |
| Species ： | confirmed as mouse by species PCR and as Mus musculus origin by COI DNA barcoding |
| Cell type ： | connective tissue fibroblasts |
| Origin ： | established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays |
| Reference(s) ： | 15132, 16798 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblasts growing as monolayer; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out initially at about 1.0-2.0 x 106 cells/25 cm2; split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 21-24 hours |
| Harvest ： | ca. 4-8 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype - 61-67, 12-16 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |