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| 产品名称： | P-815 |
|---|---|
| 商品货号： | TS258763 |
| <strong>Cell line </strong>： | P-815 |
| DSMZ no. ： | TS258763 |
| Species ： | and Mus musculus origin by COI DNA barcoding |
| Cell type ： | mastocytoma |
| Origin ： | established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system |
| Reference(s) ： | 14875, 14876, 14877, 14874, 15207 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; some cells may be adherent; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-5 x 105 cells/ml, cells grow very fast; split 1:5 to 1:10 every 3-4 days; seed out at ca. 1-2 x 106 cells/10 ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 18-22 hours |
| Harvest ： | maximum cell density at > 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype - 41(36-43)<2n> - 1-2 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |