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| 产品名称： | PEER |
|---|---|
| 商品货号： | TS258768 |
| <strong>Cell line </strong>： | PEER |
| DSMZ no. ： | TS258768 |
| Species ： | confirmed originally as human with IEF of AST, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 4-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in second relapse in 1977; cell lines BE-13 (DSM ACC 396) and PEER show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination; however, their diploid and tetraploid karyotypes indicative of earlier and later passage numbers, respectively, suggest that BE-13 is derived from PEER, rather than vice-versa; carries NUP214-ABL1 fusion gene |
| Reference(s) ： | 14574, 14608, 14884, 15867, 15868, 15217 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round cells growing mostly singly in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are very difficult to culture! Cells grow at best in 24-well-plates; seed out at ca. 1.0 x 106 cells/ml (culture should not be split during the first 3-4 days after thawing), then maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | saturation density at 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD13 -, CD19 -, CD34 -, TCRgamma/delta +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 7% polyploidy - 46(42-47)<2n>XX, der(4)?dup ins(4;4)(?p11;?q21q25), del(5)(q22q31), del(6)(q13q22), del(9)(p11p22), del(9)(q22) - resembles published karyotype - 5q-, 6q-, 9p- and 9q- associated with hematological malignancy |
| Molec. Genetics ： | expression of NKX2-5 and MEF2C confirmed by RT-PCR and Western blot |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |