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| 产品名称： | CML-T1 |
|---|---|
| 商品货号： | TS258770 |
| <strong>Cell line </strong>： | CML-T1 |
| DSMZ no. ： | TS258770 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP, later by STR-typing |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described in the literature to express T cell surface markers and to carry the BCR-ABL1 translocation e13-a2 (b2-a2) producing the p210 BCR-ABL protein. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14972, 14699, 14479, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image; image; image |
| Medium ： | 90% Iscoves MDM or RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well-plate; maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-60 hours |
| Harvest ： | maximal density at 2.5-3.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, cyCD3 +, CD3 (+), CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near tetraploid karyotype with diploid sideline - 92<4n>XXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2 |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e13-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |