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| 产品名称： | KM-H2 |
|---|---|
| 商品货号： | TS258771 |
| <strong>Cell line </strong>： | KM-H2 |
| DSMZ no. ： | TS258771 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established from the pleural effusion of a 37-year-old man with Hodgkin lymphoma (mixed cellularity progressing to lymphocyte depletion; stage IV at relapse) in 1974. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14645, 14478, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, mostly round cells in suspension, heterogeneity in cell size; bi- and polynucleated cells; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml; viability may drop to about 50%, but cells will recover within a week; then, maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:4 every second day; |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | saturation density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD13 -, CD14 +, CD15 +, CD19 -, CD21 +, CD30 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 3.5% polyploidy - 59(56-60)<3n>der(X)der(Y), -X, -1, -1, -2, -2, -3, -4, -5, -5, -6, -8, -9, -9, -10, -10, -10, -11, -12, -13, -14, -15, -15, -16, -16, -18, -19, -20, -21, -22, +25mar - add(1)(q32), add(3)(q26), add(3)(q26), del(4)(q26q31), i(4q), add(5)(p11), add(6)(q14), add(7)(p14), add(7)(q34), del(7)(q31), add(8)(q24), del(8)(p12), del(11)(q23), i(13q), der(13)t(11;13)(p13;q13), add(14)(p12)x2, del(17)(p12), i(18q) - apparently typical HD karyotype with extraordinary level of rearrangements including several HSR-bearing markers with ectopic rDNA - resembles published karyotype |
| Molec. Genetics ： | expression of fusion genes PRRC2B-MGMT (BAT2L1-MGMT) and CIITA-BX648577 confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |