微信小李
微信小章
产品简介
购买须知
| 产品名称： | U-937 |
|---|---|
| 商品货号： | TS258774 |
| <strong>Cell line </strong>： | U-937 |
| DSMZ no. ： | TS258774 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP and recently by STR fingerprinting |
| Cell type ： | histiocytic lymphoma |
| Origin ： | established from the pleural effusion of a 37-year-old man with generalized diffuse histiocytic lymphoma in 1974; cells were described in the literature to express markers and properties of monocytes; patented cell line. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14995, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal, single cells in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | saturation density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD54 +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype - 63(58-69)<3n>XXY, -2, -4, -6, +7, -9, -20, -21, +3mar, t(1;12)(q21;p13), der(5)t(1;5)(p22;q35), add(9)(p22), t(10;11)(p14;q23), i(11q), i(12p), add(16)(q22), add(19)(q13) - carries t(10;11) (seen in AML M5) - t(1;5) resembles variant of t(2;5) (histiocytic lymphoma) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |