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| 产品名称： | U-698-M |
|---|---|
| 商品货号： | TS258775 |
| <strong>Cell line </strong>： | U-698-M |
| DSMZ no. ： | TS258775 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the involved tonsil of a 7-year-old boy with lymphoblastic lymphoma at diagnosis prior to therapy in 1972; cells were described in the literature to be EBV-negative |
| Reference(s) ： | 14806, 14807, 15230 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single or clumped cells in suspension; some cells adhere to plastic |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; some cells adhere to plastic, but can be detached mechanically (trypsin not necessary) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximal density at 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 5.5% polyploidy - 49(44-50)<2n>XY, +3, +7, -14, +mar, dup(1)(q43q21.2), der(2)t(2;3)(p16;p11), add(3)(p11), del(6)(q15q22), del(9)(p22), dup(11)(q23q13), add(13)(p12), add(16)(q24) - carries large submetacentric dup(1) marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |